Natural astaxanthin enhanced antioxidant capacity and improved semen quality through the MAPK/Nrf2 pathway in aging layer breeder roosters

Table 2 Primers used for the real-time quantitative PCR

Genes	Primer sequence (5′ → 3′)	Fragment length, bp	Annealing temperature, °C	Accession number
ACTB	Forward: GCCAACAGAGAGAAGATGACAC	118	58	NM_205518
ACTB	Reverse: GTAACACCATCACCAGAGTCCA	118	58	NM_205518
SOD1	Forward: TTGTCTGATGGAGATCATGGCTTC	98	58	NM_205064
SOD1	Reverse: TGCTTGCCTTCAGGATTAAAGTGAG	98	58	NM_205064
SOD2	Forward: CAGATAGCAGCCTGTGCAAATCA	86	58	NM_204211.1
SOD2	Reverse: GCATGTTCCCATACATCGATTCC	86	58	NM_204211.1
CAT	Forward: ACCAAGTACTGCAAGGCGAAAGT	91	58	NM_001031215.2
CAT	Reverse: ACCCAGATTCTCCAGCAACAGTG	91	58	NM_001031215.2
GPX-1	Forward: TTCGAGAAGTTCCTCGTGGG	79	58	NM_0012778553.2
GPX-1	Reverse: CCTGCAGTTTGATGGTCTCG	79	58	NM_0012778553.2
GPX-4	Forward: TCAACCGTGAGGGCCAAGT	100	58	NM_001346448.1
GPX-4	Reverse: CTCGGCACGCAGCTCTAC	100	58	NM_001346448.1
Nrf2	Forward: ACATGGACAGTTCTCCTGGG	92	58	NM_205117.1
Nrf2	Reverse: CGGCTCCACAGAAGGAAGTA	92	58	NM_205117.1
ERK	Forward: AGCAAGCTTTAGCCCATCCA	108	58	NM_204150.1
ERK	Reverse: CCTTCGGCAAGTCATCCAAT	108	58	NM_204150.1
JNK1	Forward: GGGTGCATTATGGGCGAAAT	108	58	XM_421650.2
JNK1	Reverse: TTCTGGGCACGGTGTTCCTA	108	58	XM_421650.2
JNK2	Forward: AGCAGCCTCGATGCCTTGAC	110	58	AB000807.1
JNK2	Reverse: CAAGCAATTCAGGCCCAATG	110	58	AB000807.1
JNK3	Forward: CTGGTGAGTGAGCTGATGGA	82	58	NM_001318224.3
JNK3	Reverse: ACAGCAGGTAGGACATTCGT	82	58	NM_001318224.3
p38	Forward: TGTGTTCACCCCTGCCAAGT	149	58	AJ719744.1
p38	Reverse: GCCCCCGAAGAATCTGGTAT	149	58	AJ719744.1

ISSN: 2049-1891