Fig. 1

Weight loss and populations of the ileal fungi and bacteria in chickens with different severities of necrotic enteritis (NE). Chickens were sequentially infected with E. maxima and C. perfringens to induce NE and separated into five groups based on their intestinal lesion scores. NE-induced weight loss (%) A was calculated relative to mock-infected controls. The total fungal genome copy numbers B and total bacterial genome copy numbers C per gram of the ileal contents were quantified using qPCR. The percentage (%) of total fungal genome copies relative to total bacterial genome copies D was also calculated. Results were expressed as means ± SEM and individual dots represented the results of individual animals. Statistical significance (P < 0.05), denoted by different superscripts, was determined using one-way ANOVA and Tukey’s post-hoc test