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Table 2 Effect of HS and supplemental 25-hydroxycholecalciferol (25-OH-D3) on gait score (d 40) and tibia bone characteristics (d 39) of broilers

From: 25-hydroxycholecalciferol reverses heat induced alterations in bone quality in finisher broilers associated with effects on intestinal integrity and inflammation

Items

Ctrl

PF

HS

HS + 25-OH-D3

Gait score

2.10 ± 0.28

1.38 ± 0.11

1.93 ± 0.23

1.37 ± 0.12*

Bone growth

 Length, mm

104.96 ± 1.80

103.26 ± 1.47

105.30 ± 1.11

105.31 ± 1.07

 Width, mm

9.70 ± 0.23

9.96 ± 0.20

9.39 ± 0.21

9.50 ± 0.25

 Fat-free weight, g

7.53 ± 0.25

7.51 ± 0.16

7.29 ± 0.18

7.29 ± 0.15

Bone mineralization

 Ash, %Fat-free weight

45.71 ± 0.26a

46.08 ± 0.41a

43.91 ± 0.59b

45.92 ± 0.42*

 Density, g/mL

0.45 ± 0.01

0.44 ± 0.01

0.42 ± 0.01

0.45 ± 0.01*

 Cortical thickness in diaphysis, μm

25.18 ± 1.80

26.06 ± 2.39

24.32 ± 0.96

28.25 ± 1.65*

 BV/TV in diaphysis, %

0.56 ± 0.01

0.55 ± 0.01

0.54 ± 0.01

0.55 ± 0.01

 Trabecular thickness in metaphysis, μm

4.14 ± 0.05

4.17 ± 0.09

4.19 ± 0.05

4.32 ± 0.03*

 BV/TV in metaphysis, %

0.25 ± 0.01

0.27 ± 0.03

0.24 ± 0.01

0.25 ± 0.01

Bone mechanical properties

 Fracture load, N

708.33 ± 23.86a

725 ± 28.14a

587.5 ± 33.74b

707.14 ± 33.5*

 Yield load, N

710.65 ± 77.63

678.56 ± 42.13

594.27 ± 51.06

769.00 ± 69.67

 Slope, N/mm

310.91 ± 62.24

324.98 ± 82.38

296.30 ± 24.34

472.13 ± 50.13*

 AUC, N × mm × 103

3.36 ± 0.36

3.14 ± 0.39

2.63 ± 0.21

3.15 ± 0.34

  1. Data represent means with standard error;
  2. Treatments Ctrl, PF and HS without common superscript are significantly different at P < 0.05. * denotes significant difference between HS and HS + 25-OH-D3 at P < 0.05;
  3. AUC Area under the load-displacement curve, BV/TV Bone volume/Total volume