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Fig. 8 | Journal of Animal Science and Biotechnology

Fig. 8

From: NORHA, a novel follicular atresia-related lncRNA, promotes porcine granulosa cell apoptosis via the miR-183-96-182 cluster and FoxO1 axis

Fig. 8

NORHA and oxidative stress synergistically induce GC apoptosis. a H2O2 induces oxidative stress. Porcine GCs were treated with H2O2 (0, 500, and 1,000 μmol/L) for 90 min, and reactive oxygen species (ROS) levels were detected by flow cytometry. b, c Oxidative stress induced by H2O2 induces GC apoptosis. The apoptosis rate (b) and protein levels of T-CASP3 and C-CASP3 (c) were measured in GCs treated with 1,000 μmol/L H2O2. d NORHA and oxidative stress synergistically induced GC apoptosis. GCs were treated with pcDNA3.1-NORHA with or without H2O2, and the apoptosis rate was calculated. The data are presented as the mean ± SEM of at last three independent experiments. * P < 0.05. ** P < 0.01

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