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Fig. 6 | Journal of Animal Science and Biotechnology

Fig. 6

From: NORHA, a novel follicular atresia-related lncRNA, promotes porcine granulosa cell apoptosis via the miR-183-96-182 cluster and FoxO1 axis

Fig. 6

FoxO1 is a common target of the miR-183-96-182 cluster in GCs. a The common putative targets of the miR-183-96-182 cluster. The putative targets of miR-183, miR-96, and miR-182 were predicted by five programs: TargetScan, Pictar2, PITA, RNA22, and RNAhybrid. b The MFE of the miR-183-96-182 cluster binding to the 3′-UTR of the porcine FoxO1 gene was predicted by RNAhybrid. c The binding sites of the miR-183-96-182 cluster in the 3′-UTR of the porcine FoxO1 gene. d, e Luciferase assay. Luciferase activity was measured in HEK293T cells cotransfected with mimics (mi) of miR-183, miR-96, or miR-182 and reporter vectors of FoxO1 3′-UTR harboring the wild-type (d) or mutated (e) miRNA-binding site. f, g GCs were transfected with mimics (mi) of miR-182, miR-96, or miR-183, and the mRNA (f) and protein (g) levels of FoxO1 were detected by qRT-PCR and western blotting, respectively. The data are presented as the mean ± SEM. of at last three independent experiments. * P < 0.05. ** P < 0.01. NS, not significant

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