Fig. 8

Western blotting evaluation of the expression of hepatic autophagy and apoptosis signaling proteins in the ilea tissues of piglets of the three treatment groups. A-F: LC3, LC3-II/LC3-I, Atg5, Beclin-1, Bax, SOD2, respectively. CON, control piglets orally inoculated with PBS; LPS, piglets received intraperitoneal injection (i.p.) of LPS (25 μg/kg body weight); ZJ617 + LPS, piglets orally inoculated with ZJ617 (1 × 10¹⁰ CFU/d) for 2 weeks before i.p. injection of LPS. The protein bands were quantified by densitometry analysis and normalized to the level of GAPDH. Values are shown as means ± SEs, n = 6. Labeled means without a common letter differ, P < 0.05. Atg5, autophagy-related protein 5; Bax, B-cell lymphoma-2 associated X protein; LC3-II, microtubule-associated protein 1 light chain 3; SOD2, superoxide dismutase protein 2