Fig. 5
Western blotting evaluation of the expression of ilea tight junction proteins in piglets of the three treatment groups. A-C: ZO-1, Cluaudin-3, Occludin, respectively. CON, control piglets orally inoculated with PBS; LPS, piglets received intraperitoneal injection (i.p.) of LPS (25 μg/kg body weight); ZJ617 + LPS, piglets orally inoculated with ZJ617 (1 × 1010 CFU/d) for 2 weeks before i.p. injection of LPS. ZO-1, zonula occludens-1. The protein bands were quantified by densitometry analysis and normalized to the level of GAPDH. Values are shown as means ± SEs, n = 6. Labeled means without a common letter differ, P < 0.05