Fig. 3

Western blotting evaluation of the expression of cell signaling molecules in the ilea of piglets of the three treatment groups. A-C: p-p38/p38, p-JNK, IκBα, respectively. CON, control piglets orally inoculated with PBS; LPS, piglets received intraperitoneal injection (i.p.) of LPS (25 μg/kg body weight); ZJ617 + LPS, piglets orally inoculated with ZJ617 (1 × 10¹⁰ CFU/d) for 2 weeks before i.p. injection of LPS. The protein bands were quantified by densitometry analysis and normalized to the level of GAPDH. Values are shown as means ± SEs, n = 6. Labeled means without a common letter differ, P < 0.05. IκBα, nuclear factor-kappa B inhibitor alpha; p-, phospho-; p38, mitogen-activated protein kinase (MAPK) p38; JNK, c-Jun N-terminal kinase