Fig. 2
From: Dietary calcium supplementation promotes the accumulation of intramuscular fat
Calcium addition increases the triglyceride content of C2C12 cells. A Oil red O staining. B Triglyceride content. C Cytoplasm, mitochondria and endoplasmic reticulum calcium levels. D Mitochondrial staining. E Fluorescence intensity of mitochondrial staining. F Mitochondrial DNA quantification. G Changes of calcium levels in the cytoplasm, mitochondria and endoplasmic reticulum with or without chelation treatment (BAPTA, 0.1 μmol/L). H Intracellular triglyceride (TG) levels treated with different concentrations of calcium, with or without chelation. I Changes of calcium levels in the cytoplasm and mitochondria (10 nmol/L Ru360). J Changes of calcium levels in the cytoplasm and mitochondria (10 nmol/L Mitoxantrone). K Triglyceride content. L Acyl-CoA dehydrogenase activity. M Western blot of mitochondrial membrane proteins and redox proteins. The data are expressed as the mean ± SD. *P < 0.05, **P < 0.01