Fig. 1
From: Dietary calcium supplementation promotes the accumulation of intramuscular fat
Effect of calcium on the differentiation and proliferation of C2C12 cells. A Cell counting assay (CCK-8 assay). B mRNA expression in C2C12 cells before and after differentiation. C mRNA expression of genes associated with regulation of slow-twitch fibers (MEF2C, TNNI1, PGC-1α, MYH7, TNNC1) and fast-twitch fibers (MYH1, MYH4, TNNC2, TNNI2), respectively. D, E, F and G Immunofluorescence analysis and quantification of myosin heavy chain (MYHC), slow-twitch myosin (Slow-MYHC) and fast-twitch myosin (Fast-MYHC) signals in C2C12 cells treated with different concentrations of calcium. H Effects of different concentrations of calcium on cell proliferation. I Wound-healing assay. 2 mmol/L, normal calcium level; 3 mmol/L, calcium supplementation. The data are expressed as the mean ± SD. *P < 0.05, **P < 0.01