Fig. 8

CircCPE serve as a mir-138 sponge to attenuate its inhibitory of FOXC1. (A) miR-138 mimics were co-transfected with pCK-FOXC1-WT or pCK- FOXC1- mut) into HEK293T cells. Luciferase activities were measured 24 h after transfection. Renilla luciferase activity was normalized to Firefly luciferase activity. (B) The pCK-FOXC1-WT was co-transfected with the miR-138 mimic and 1× or 2× pcD2.1-circCPE into HEK293T cells, and luciferase activities were measured after transfection. (C) The expression of FOXC1 was detected by real-time qPCR and western blots after transfected with miR-138 mimics or co-transfected with circCPE or circCPE alone in bovine myoblasts. (D) The correlation analysis in expression of FOXC1 and circCPE during myoblast differentiation (R² = 0.84). (E) Effect of upregulated circCPE accompanied by suppressing FOXC1 on the mRNA and protein levels of proliferation-related gene. (F) Effect of upregulated circCPE accompanied by suppressing FOXC1 on the mRNA and protein levels of apoptosis maker genes. (G) Effect of upregulated circCPE accompanied by suppressing FOXC1 on the mRNA and protein levels of differentiation marker genes. Values are means ± SEM for three individuals. P < 0.05, P < 0.01. (H) Schematic illustration of circCPE competitively binding miR-138 in regulating myoblast proliferation, apoptosis and differentiation