Fig. 7
From: The circular RNA circCPE regulates myoblast development by sponging miR-138
CircCPE inhibited myoblasts apoptosis and differentiation in a miR-138 dependent manner. (A and B) The expression of apoptosis marker genes was detected by real-time qPCR and western blots after transfection with pCD2.1-NC + mimic NC, pCD2.1-NC + miR-138, pCD2.1- circCPE +miR-138 and pCD2.1-circCPE +mimic NC in bovine myoblasts. (C and D) The expression of differentiated marker genes was detected by real-time qPCR and western blots after after transfection with pCD2.1-NC + mimic NC, pCD2.1-NC + miR-138, pCD2.1- circCPE +miR-138 and pCD2.1-circCPE +mimic NC in bovine myoblasts. (E) Cell differentiation was detected by immunofluorescence (MyHC) after transfected with miR-138 mimics or co-transfected with circCPE or circCPE alone. The images were observed under a fluorescence microscope (scale bar indicates 1000 μm). Values are means ± SEM for three individuals. P < 0.05, P < 0.01