Fig. 3
From: The circular RNA circCPE regulates myoblast development by sponging miR-138
Overexpression of circCPE inhibited the apoptosis and differentiation of myoblasts. (A and B) The expression of apoptotic marker genes were detected using real-time qPCR and western blots after transfection with pcD2.1-circCPE vector. (C) Annexin V-APC/7AAD staining followed by flow cytometry revealed that circCPE inhibit myoblasts apoptosis. (D and E) The expression of differentiated marker genes was detected by qRT-PCR and western blot after transfection with pcD2.1-circCPE vector and culturing for 3 d. (F) Cell differentiation was detected by immunofluorescence (MyHC) and observed under a fluorescence microscope. Scale bar indicates 1,000 μm. Values are means ± SEM for three individuals. P < 0.05, P < 0.01