Specific inhibition of Streptococcus bovis by endolysin LyJH307 supplementation shifts the rumen microbiota and metabolic pathways related to carbohydrate metabolism

Table 1 PCR primers used in quantitative real-time PCR

Target species	Primer	Sequence (5’ → 3’)	Size, bp	Efficiency^a	References
General bacteria	F	CGGCAACGAGCGCAACCC	130	1.975	[28]
General bacteria	R	CCATTGTAGCACGTGTGTAGCC	130	1.975	[28]
Ciliate protozoa	F	GCTTTCGWTGGTAGTGTATT	223	1.872	[29]
	R	CTTGCCCTCYAATCGTWCT
	R	CCCATCCTATAGCGGTAAACCTTTG
Streptococcus bovis	F	TTCCTAGAGATAGGAAGTTTCTTCGG	127	1.944	[30]
Streptococcus bovis	R	ATGATGGCAACTAACAATAGGGGT	127	1.944	[30]

ISSN: 2049-1891