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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: TLR7/8 signalling affects X-sperm motility via the GSK3 α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos

Fig. 5

The percentage of Y-sperm and X-sperm in the upper layer and lower layer sperm after TLR7/8 agonists treatment. a, comparison of sperm motility, membrane integrity, and acrosome integrity in the upper layer and lower layer sperm with 1 μmol/L R848 for 30 min (n = 5). b, sperm were stained using SYBR-14/PI. White triangle indicates membrane integrity; yellow arrows indicates membrane damaged (Bars = 20 μm, n = 5). c, sperm were stained using FITC-PNA. White triangle indicates acrosome integrity; yellow arrows indicates acrosome damaged (Bars = 20 μm, n = 5). d, localisation of TLR8 in the upper layer and lower layer goat sperm after incubate with 1 μmol/L R848 for 30 min. Yellow arrows represent TLR8 positive sperm, white arrows represen TLR8 negative sperm (Bars = 50 μm). e, the percentage of X-sperm and Y-sperm in the upper layer and lower layer sperm were measured using flow cytometer system, values are the mean ± SEM of three replicates. *P < 0.05 compared with the control

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891