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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: TLR7/8 signalling affects X-sperm motility via the GSK3 α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos

Fig. 3

The percentage and localisation of TLR8 in goat testes, epididymis and sperm. a, localisation of TLR8 in goat testes at 8 months age. Cross-sections of goat testes were stained with TLR8 (red) or PNA (green), a marker of acrosome in round spermatids and sperm. Yellow arrows represent PAN+ and TLR8+ positive cells, white arrows represent PAN+ and TLR8− positive cells. Scale bar indicates 20 μm. b, localisation of TLR8 in goat epididymis at 8 months age. Cross-sections of epididymis were stained with TLR8 (red) or PNA (green). Scale bar indicates 50 μm. c, localisation of TLR8 in goat sperm collected by ejaculation, TLR8+ signals (red) were mainly localised at the connecting piece and midpiece of the sperm tail. Scale bar indicates 20 μm. Yellow arrows represent TLR8 positive sperm (P), white arrows represent TLR8 negative sperm (N). d, the percentage of TLR8 positive/negative cell in goat testis. e, the percentage of TLR8 positive/negative sperm in goat epididymis. f, the percentage of TLR8 positive/negative sperm in goat sperm. Bars represent the mean ± SEM (n = 3)

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891