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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: TLR7/8 signalling affects X-sperm motility via the GSK3 α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos

Fig. 2

The percentage and localisation of TLR7 in goat testes, epididymis and sperm. a, localisation of TLR7 in goat testes at 8 months age. Cross-sections of goat testes were stained with TLR7 (red) or PNA (green), a marker of acrosome in round spermatids and sperm. Yellow arrows represent PAN+ and TLR7+ positive cells, white arrows represent PAN+ and TLR7− positive cells. Scale bar indicates 20 μm. b, localisation of TLR7 in goat epididymis at 8 months age. Cross-sections of epididymis were stained with TLR7 (red) or PNA (green). Scale bar indicates 50 μm. c, localisation of TLR7 in goat sperm collected by ejaculation, TLR7+ signals (red) were observed at the entire tail of the sperm. Scale bar indicates 20 μm. Yellow arrows represent TLR7 positive sperm (P), white arrows represen TLR7 negative sperm (N). d, the percentage of TLR7 positive/negative cell in goat testis. e, the percentage of TLR7 positive/negative sperm in goat epididymis. f, the percentage of TLR7 positive/negative sperm in goat sperm. Bars represent the mean ± SEM (n = 3)

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891