Table 3 Sequences of the primers designed to qMSP reaction by using MethPrimer tool
Gene | Primers sequence (5'→3') | GC% | Amplicon size, bp | NCBI no. | |
|---|---|---|---|---|---|
CD72 | M | F: AACGGGTTATGTGTCGTTATTAGTC R: AAACTAAACCCTACTACCTTCTCGC | 60.00 72.00 | 107 | 395923 |
U | F: TGGGTTATGTGTTGTTATTAGTTGT R: ACTAAACCCTACTACCTTCTCACA | 64.00 70.83 | 103 | ||
CXCR5 | M | F: AGAGGTTGGGATTTACGGTAATAAC R: ACAACTTTCTACCTTTACAAACGCT | 56.00 56.00 | 156 | 419784 |
U | F: AGGTTGGGATTTATGGTAATAATGT R: ACAACTTTCTACCTTTACAAACACT | 56.00 56.00 | 154 | ||
NFATC1 | M | F: CGATTCGGAAATATTAATAAAGC R: AAAAATAATATAAACCCTACCCGAC | 52.17 60.00 | 100 | 420815 |
U | F: TTTGTATTGATTTGGAAATATTAATAAAGT R: ACAAAAATAATATAAACCCTACCCAAC | 46.67 62.96 | 109 | ||
SYK | M | F: TATTAGGCGTTTTCGGGAAC R: AAATTAATACATTTACTCGCCGCT | 70.00 54.17 | 115 | 427272 |
U | F: GTTTATTAGGTGTTTTTGGGAATGA R: CCAAATTAATACATTTACTCACCACT | 68.00 57.69 | 120 | ||
CYR61 | M | F: TTTGGTTTTAGTGTTTAAAGACGT R: TTATATTTACCTTCAAAAAAACGTA | 58.33 44.00 | 150 | 429089 |
U | F: TTTTGGTTTTAGTGTTTAAAGATGT R: TATTTATATTTACCTTCAAAAAAACATA | 56.00 42.86 | 154 | ||
NR4A3 | M | F: GGGAAAGGATAAAGTTTTTGTAGTC R: AAACTCAAACGTAACCCTAAACGTA | 52.00 56.00 | 179 | 420996 |
U | F: GGGAAAGGATAAAGTTTTTGTAGTTG R: AAACTCAAACATAACCCTAAACATA | 53.85 56.00 | 179 | ||
SERPING1 | M | F: GGTAACGAGAGTTTGGATTTGTAAC R: CCTAAATAAACCCTAAAAACTACGC | 56.00 64.00 | 163 | 423132 |
U | F: TGGTAATGAGAGTTTGGATTTGTAAT R: CTAAATAAACCCTAAAAACTACACC | 53.85 64.00 | 163 | ||
TNFRSF14 | M | F: GTTTTAGTTATTTTTGTTTTTACGTTCGT R: CCGCTATCACTATACAACTTCTCG | 65.52 62.50 | 298 | 420403 |
U | F: AGTTATTTTTGTTTTTATGTTTGT R: CACTATCACTATACAACTTCTCACC | 62.50 64.00 | 292 | ||
IKZF1 | M | F: GTAGTAGTAATTGTTGGAGGAGGC R: AAAAATAACTTTACGAAACAACGAA | 62.50 64.00 | 192 | 395974 |
U | F: GTAGTAGTAATTGTTGGAGGAGGTG R: AAAAATAACTTTACAAAACAACAAA | 64.00 64.00 | 192 | ||
KLHL6 | M | F: TTTTTTGGATAATGAGTGTTTAACG R: AAACACCAAAAAAAATCCCGTA | 52.00 63.64 | 100 | 424762 |
U | F: TTTTTGGATAATGAGTGTTTAATGA R: CTAAAACACCAAAAAAAATCCCATA | 48.00 64.00 | 102 | ||
ANGPTL4 | M | F: TAATTTTAACGGGAAGTATTTTCGT R: CAACTTTAAAACTCTACCTCCAACG | 56.00 60.00 | 156 | 769087 |
U | F: TAATTTTAATGGGAAGTATTTTTGT R: ACTTTAAAACTCTACCTCCAACACA | 56.00 60.00 | 154 | ||