Table 2 Sequences of real-time PCR primers
Genes | Primer sequence (5′→3′) | Accession no. |
|---|---|---|
Claudin-1 | F: AAGTGCATGGAGGATGACCA | NM_001013611.2 |
| Â | R: GCCACTCTGTTGCCATACCA | Â |
Occludin | F: TCATCGCCTCCATCGTCTAC | NM_205128.1 |
| Â | R: TCTTACTGCGCGTCTTCTGG | Â |
ZO-1 | F: TATGAAGATCGTGCGCCTCC | XM_015278981.1 |
| Â | R: GAGGTCTGCCATCGTAGCTC | Â |
Mucin-2 | F: AGCGAGATGTTGGCGATGAT | NM_001318434.1 |
| Â | R: AAGTTGCCACACAGACCACA | Â |
IL-1β | F: ACTGGGCATCAAGGGCTA | NM_204524 |
| Â | R: GGTAGAAGATGAAGCGGGTC | Â |
IL-8 | F: GGCTTGCTAGGGGAAATGA | AJ009800 |
| Â | R: AGCTGACTCTGACTAGGAAACTGT | Â |
IL-10 | F: CGCTGTCACCGCTTCTTCA | NM_001004414.2 |
| Â | R: CGTCTCCTTGATCTGCTTGATG | Â |
TNF-α | F: GAGCGTTGACTTGGCTGTC | NM_204267 |
| Â | R: AAGCAACAACCAGCTATGCAC | Â |
IFN-γ | F: AAAGCCGCACATCAAACACA | NM_205149.1 |
| Â | R: GCCATCAGGAAGGTTGTTTTTC | Â |
TLR-4 | F: CCACTATTCGGTTGGTGGAC | NM_001030693.1 |
| Â | R: ACAGCTTCTCAGCAGGCAAT | Â |
β-actin | F: GAGAAATTGTGCGTGACATCA | L08165 |
| Â | R: CCTGAACCTCTCATTGCCA | Â |