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Table 2 Primers for miRNA quantitative real-time PCR

From: Bta-miR-34b controls milk fat biosynthesis via the Akt/mTOR signaling pathway by targeting RAI14 in bovine mammary epithelial cells

Genes

Primer sequence (5'→3')

Annealing temperature

U6

F: GCTTCGGCAGCACATATACT

R: TTCACGAATTTGCGTGTCAT

61 °C

miR-34b

GGGGAGGCAGTGTAATTAGCTGATTG

61 °C

  1. F forward, R reverse
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891