Table 1 Primers for mRNA quantitative real-time PCR
Genes | Primer sequence (5'→3') | Annealing temperature |
|---|---|---|
UXT | F: TAGCCACCCTCAAGTATGTTCG R: CGAGGTAGGAGGACAGGAGT | 61 °C |
PPARγ | F: AAAGGAGAGCCTGAACTTGGAG R: TCTGAACTGTGCTGTGGCAA | 61 °C |
FASN | F: CCCTGAATGTGAGGCAGTGTG R: TTAGCTGTGGTGAGGAGCCA | 61 °C |
FABP4 | F: GGACAGCAAGGAGACCTACAA R: GTTTGAGATGTTCGGGGAGGA | 61 °C |
CEBPα | F: CGAAGTGTAGGAACCGGCGA R: CACAAACTCCAGACGTTCCTTC | 61 °C |
CEBPβ | F: TGGTGAATAGTGCTGCCCAT R: GGTGGTAGTTGTGGAAGCCC | 61 °C |
Akt | F: TCACCATTACGCCACCTGAC R: TCCTCTCCATCCTGTGTTGG | 61 °C |
RAI14 | F: AAGCTCCACCACCTCCTATCA R: GTATGGAACTAATCTCAGCCTTGAA | 61 °C |
mTOR | F: TGGACACCAACAAGGACGAC R: TCCCACTGACCTAAACCCCA | 61 °C |
4E-BP1 | F: GTTCCTGATGGAGTGTCGGA R: AACTGTGACTCTTCACCGCCT | 61 °C |
P70S6K | F: ATCACCAAGGTCACGTCAAAC R: TGCTCCCAAACTCCACCAAT | 61 °C |
S100G | F: CTCCAGAAGAACTGAAGGGCA R: CAAAAAGCTCATCGAGGGTGC | 61 °C |
PABPC1 | F: GGTGCCAGACCTCATCCATT R: TCGACATGACTCGTGGAACC | 61 °C |
TFPI2 | F: ATGGTAACACAGGCAAGCGA R: AAATCAGGGAGAGCAACCCC | 61 °C |
PRICKLE1 | F: GCGCCGGAAGGAATCGAAC R: CAGCCCGAGTCATCATCCG | 61 °C |
SYNE2 | F: GTGGTTTGCAGCTTTCCGAG R: CTGTTGGTCTTCAGCGGGAA | 61 °C |
IGF2 | F: GACCTTGAGGACGAGGAGGT R: CTGAGCAGGTGGGGATCAAG | 61 °C |
IVNS1ABP | F: AGGCAAAGAGGCACATGGGA R: GCCACCAGGCAAACTGATTTC | 61 °C |
SGK1 | F: TCTGGCGATGACGGTGAAAAC R: TCATTCAGGCCCATCCTTCTC | 61 °C |
PLSCR2 | F: ACCAAAGAAGGACACACTTAGAGA R: CACTTGAGGGATCTGAAAACTACT | 61 °C |
TNS1 | F: ACCAGCTTCTTGCCATCCAC R: CGGAGCAGTCTCAGTGTTGA | 61 °C |
MDH1 | F: CTGCAAAGGCCATCTGTGAC R: ACGGGGAACGAGTAAAGCAG | 61 °C |
S100A10 | F: TAATCGCTGGGCTCACCATC R: AAGCTGTGGGACAGAGTCCT | 61 °C |
TM4SF1 | F: CGATCCTCTTGGCACTTGGT R: TGGCGAGAGGAACAATGACC | 61 °C |
PSPH | F: CCCCTTGCAGTCGGAACTT R: TGGATGACGGTGCTATCGAC | 61 °C |
BTBD11 | F: GGCCCGAACCTGTAAACTGT R: TCTGCAACAGCATCTCAGGG | 61 °C |
SYT11 | F: CCACCAGATACCTTCCCCCT R: GGTGATCTCAGCCATGTCGT | 61 °C |
TCIM | F: CCGAAGAATCCCCCATCCAT R: GCCCACAGCTTTCTTACGTG | 61 °C |
TAGLN2 | F: ACTCTCCGACCCACGTGAA R: TGCCATCCTTGAGCCAGTT | 61 °C |
RCN1 | F: TGTCAGAACGGGAGCAGTTT R: GTGGTCGTAGTCTTGGGGAAG | 61 °C |
NDFIP2 | F: AGAAACAGAGGCGGAAGAGTC R: CTGGTGGTGATCCATCCTGC | 61 °C |
ANXA5 | F: GCCATGGCACAGGTTCTCAG R: TGCGGGATGTCAACAGAGTC | 61 °C |
TRPM6 | F: TGCAGGTGCCAGTCATAACA R: CAGGATGTCGTTCAGCTCCTT | 61 °C |
RCAN1 | F: TAGCTCCCTGATTGCCTGTGT R: ATGTAGCTGGAGTCTGGCATC | 61 °C |
COL5A2 | F: TGGGGCACTATGATGACAGC R: ATCATCACAAGTCCGGGCAG | 61 °C |