Fig. 7

RAI14 is the target gene of miR-34b in BMECs. a, b The relative mRNA and protein expression levels of RAI14 in BMECs following transfection with a miR-34b mimic or inhibitor. c The BMECs were co-transfected with the miR-34b mimic and a luciferase reporter containing a fragment of the RAI14 3′-UTR harboring either the miR-34b binding site (RAI14–3′-UTR WT) or a mutant (RAI14–3′-UTR MUTA). d Target site of miR-34b in the RAI14 3′-UTR and the construction of the luciferase (Luc) expression vector fused with the RAI14 3′-UTR. Values are presented as the mean ± SEM. *, P < 0.05. **, P < 0.01. Rluc = Renilla luciferase, fluc = firefly luciferase, WT = wild-type, MUTA = mutation type, NC = negative control