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Table 5 Effects of resveratrol on the expression of genes and proteins related to tight junctions and immunity in the jejunal mucosa of weaned piglets challenged with deoxynivalenol a

From: Dietary resveratrol attenuation of intestinal inflammation and oxidative damage is linked to the alteration of gut microbiota and butyrate in piglets challenged with deoxynivalenol

Variable

Treatments2

SEM

P-value

CON

DON

RES

DON+RES

DON

RES

RES × DON

Gene expressions

 Claudin-1

1.01ab

0.69c

1.18a

0.84bc

0.07

< 0.001

0.040

0.886

 ZO-1

1.03b

0.52c

1.96a

0.90b

0.09

< 0.001

< 0.001

0.004

 Occludin

1.02b

0.62c

1.41a

0.98b

0.12

0.005

0.002

0.936

 TNF-α

1.03bc

2.08a

0.92c

1.37b

0.12

< 0.001

0.002

0.019

 TGF-β

1.01b

0.32d

1.19a

0.51c

0.06

< 0.001

0.004

0.941

 IL-1β

1.02c

3.30a

0.91c

1.86b

0.15

< 0.001

< 0.001

< 0.001

 IL-6

1.03c

1.76a

0.86c

1.44b

0.09

< 0.001

0.014

0.386

 IL-8

1.02ab

1.24a

0.93b

1.13ab

0.08

0.011

0.198

0.875

The cytokines levels, ng/g protein

 IL-1β

41.35c

69.45a

36.45c

52.68b

2.77

< 0.001

< 0.001

0.041

 IL-6

45.19b

58.77a

40.67b

53.60a

2.25

< 0.001

0.040

0.888

 TNF-α

7.59c

11.44a

6.94c

8.88b

0.41

< 0.001

< 0.001

0.026

Protein expressions

 Claudin-1/β-actin

1.01b

0.38d

1.37a

0.72c

0.06

< 0.001

< 0.001

0.900

 ZO-1/β-actin

0.71b

0.30d

0.98a

0.46c

0.02

< 0.001

< 0.001

0.018

 Occludin//β-actin

0.76a

0.23c

0.99a

0.48b

0.08

< 0.001

0.012

0.847

 Claudin-3/β-actin

1.33b

0.66c

1.96a

0.99bc

0.13

< 0.001

0.006

0.293

  1. aValues are means and standard error of the means, n = 8 per treatment; labeled means in a row without a common letter differ, P ≤ 0.05; CON Control, DON Deoxynivalenol, DON+RES Combination of deoxynivalenol and resveratrol, IL-1β Interleukin 1 beta, IL-6 Interleukin 6, IL-8 Interleukin 8, RES Resveratrol, SEM Standard error of the mean, TGF-β Transforming growth factor beta, TNF-α Tumor necrosis factor alpha, ZO-1 Zonula occludens-1