Skip to main content
Fig. 7 | Journal of Animal Science and Biotechnology

Fig. 7

From: Pyrroloquinoline quinone regulates the redox status in vitro and in vivo of weaned pigs via the Nrf2/HO-1 pathway

Fig. 7

Effects of PQQ on tight junctions expression and cell viability in cells treated with siRNA. a Western blot analysis of ZO-1, ZO-2, Occludin and Claudin-1 in IPEC-J2 cells treated with or without Nrf2 siRNA-2. The densitometric values were normalized to those of β-actin. n = 3. b Viability of IPEC-J2 cells treated with or without Nrf2 siRNA-2 and incubated in the presence or absence of H2O2 or PQQ. CTRL, cells cultured with basal medium; H2O2, cells cultured with 200 μmol/L H2O2 for 2 h; PQQ, cells cultured with 10 nmol/L PQQ for 6 h and then incubated with both H2O2 (200 μmol/L) and PQQ (10 nmol/L) for another 2 h. NC siRNA, cells transfected with NC siRNA and then pretreated with PQQ for 6 h prior to incubation with both PQQ and H2O2 for another 2 h. Nrf2 siRNA, cells transfected with Nrf2 siRNA-2, pretreated with PQQ for 6 h, and then incubated with both PQQ and H2O2 for another 2 h. n = 6. * denotes a significant difference (P < 0.05) with respect to the CTRL group. # denotes a significant difference (P < 0.05) with respect to the H2O2 group

Back to article page