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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Pyrroloquinoline quinone regulates the redox status in vitro and in vivo of weaned pigs via the Nrf2/HO-1 pathway

Fig. 1

Effects of PQQ and H2O2 on cell viability in vitro. a, Cells were incubated with H2O2 for 2, 6, 18 and 24 h at concentrations of 0, 200 μmol/L, 500 μmol/L, 800 μmol/L, 1 mmol/L, 1.5 mmol/L and 2 mmol/L; b, Cells were incubated with PQQ at concentrations of 1 nmol/L, 10 nmol/L, 100 nmol/L, 1 μmol/L, 10 μmol/L and 100 μmol/L for 6, 18, 36 and 48 h; c, Cells were pretreated with PQQ for 6 h at concentrations of 1 nmol/L, 10 nmol/L, or 100 nmol/L and were then treated with both H2O2 (200 μmol/L) and PQQ for another 2 h. Cell viability in all treatment groups were evaluated with a CCK-8 assay. CTRL, cells cultured in basal medium; H2O2, cells cultured with 200 μmol/L H2O2; PQQ, cells cultured with 10 nmol/L PQQ for 6 h and then incubated with both 200 μmol/L H2O2 and 10 nmol/L PQQ for another 2 h. * denotes a significant difference (P < 0.05) with respect to the CTRL group. # denotes a significant difference (P < 0.05) with respect to the H2O2 group. n = 6

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