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Table 1 Primer sequences and optimal annealing temperatures (OAT) of genes selected for real-time PCR

From: Protective effects of sodium butyrate on rotavirus inducing endoplasmic reticulum stress-mediated apoptosis via PERK-eIF2α signaling pathway in IPEC-J2 cells

Name

Sequence (5′→3′)

OAT, °C

GenBank ID

RV-QF

TCAGTTCGTCAGGAATATGC

53.5

AF317123

RV-QR

CTTGAAGGTGAGTAGTTGGT

  

GPR109a-QF

ATGCTGGACCCTTTGGTGTAT

56.4

XM021072989

GPR109a-QR

GGCTTGTGCTGCGGTTATT

  

GRP78-QF

TCGGCGATGCAGCCAAGAAC

59.8

XM001927795

GRP78-QR

CGGGTCATTCCATGTCCGGC

  

PERK-QF

CTGCCACTTCAGCATCATTC

61.7

XM021086085

PERK-QR

TTCCATCCAGGTCACCACAT

  

IRE1-QF

CGTCCTGGATCCAAAACT

54

XM005668695

IRE1-QR

GTCAGATAGCGCAGGGTCTC

  

ATF6-QF

CCGAAGAGAAGAGCCATCTG

60.3

XM021089515

ATF6-QR

TCCTTTGATTTGCAGGGTTC

  

CHOP-QF

CACTCTTGACCCTGCCTCTC

58.4

NM001144845

CHOP-QR

GACTGGAATCAGGCGAGTGT

  

Bcl-2-QF

TGTGTGTGGAGAGCGTCAACC

62.5

XM021099593

Bcl-2-QR

CAGAGACAGCCAGGAGAAATCAA

  

Bax-QF

CCACCAGCTCTGAGCAGATCA

61.3

XM003127290

Bax-QR

GCCGCCACTCGGAAAAA

  

Caspase9-QF

GTCTGCCCACACCTAGTGAC

61.7

XM003127618

Caspase9-QR

AGGGGTCCCAGCCTCATTAT

  

Caspase3-QF

TGGCGTGTCAGAAAATACCAGT

60.5

NM214131

Caspase3-QR

GATCCGTCCTTTGAATTTCGCC

  

β-actin-QR

TCTGGCACCACACCTTCT

59.0

U07786

β-actin-QF

TGATCTGGGTCATCTTCTCAC