Fig. 8

SB alleviated RV induced apoptosis by regulating PERK-eIF2α signaling pathway via GPR109a in IPEC-J2 cells. The IPEC-J2 were transfected with constructed GPR109a siRNA1. Then, cells were pretreated with SB (4 mmol/L) for 24 h, followed by challenge with or without RV (10 MOI) for 1 h, and then incubated with SB (4 mmol/L) for a further 24 h. a Protein expressions of t-PERK, p-PERK, t-eIF2α, p-eIF2α, pro-caspase9, cle-caspase9, pro-caspase3, and cle-caspase3 were determined by western blotting. b Results were expressed as the ratio of p-PERK and t-PERK, p-eIF2α and t-eIF2α, pro-caspase9 and β-actin, cle-caspase9 and β-actin, pro-caspase3 and β-actin, and cle-caspase3 and β-actin protein levels. Equal loading was monitored with anti-β-actin antibody. ^*P < 0.05, ^**P < 0.01, data are expressed as means ± S.D. from three independent experiments at least