Fig. 7

Effect of SB on ERS-mediated apoptosis related gene relative mRNA expressions in RV-infected IPEC-J2 cells after GPR109a knockdown. IPEC-J2 were transfected with constructed GPR109a siRNA1. Then, cells were pretreated with SB (4 mmol/L) for 24 h, followed by challenge with or without RV (10 MOI) for 1 h, and then incubated with SB (4 mmol/L) for a further 24 h. The GRP78, PERK, eIF2α, ATF4, CHOP, Bcl2, caspase9, and caspase3 mRNA expressions were measured by qRT-PCR