Fig. 6

Protective effects of SB on RV induced apoptosis in IPEC-J2 cells. The IPEC-J2 cells were pretreated with SB (0, 1, 2, 4, 8, 16 mmol/L) for 24 h, followed by challenge with or without RV (10 MOI) for 1 h, and then incubated with SB (0, 1, 2, 4, 8, 16 mmol/L) for a further 24 h. a GPR109a mRNA expression was measured by qRT-PCR. b IPEC-J2 were transfected with constructed GPR109a small interfering RNAs (GPR109a siRNA1 and siRNA2) or its negative control (siCtrl). qRT-PCR was used to assess the mRNA expression of GPR109a. c IPEC-J2 were transfected with constructed GPR109a siRNA1. Then, cells were pretreated with SB (4 mmol/L) for 24 h, followed by challenge with or without RV (10 MOI) for 1 h, and then incubated with SB (4 mmol/L) for a further 24 h. c GPR109a mRNA expression was measured by qRT-PCR. d Apoptotic populations of cells double stained with PI- and FITC-labeled Annexin V were measured by flow cytometry. ^*P < 0.05, ^**P < 0.01, data are expressed as means ± S.D. from three independent experiments at least