Skip to main content
Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Protective effects of sodium butyrate on rotavirus inducing endoplasmic reticulum stress-mediated apoptosis via PERK-eIF2α signaling pathway in IPEC-J2 cells

Fig. 3

The PERK inhibitor (GSK) alleviated RV induced apoptosis by regulating PERK-eIF2α signaling pathway in IPEC-J2 cells. Cells were pre-treated with the PERK inhibitor (GSK, 1 or 10 μmol/L) for 24 h, followed by challenge with RV (10 MOI) for 1 h, and then incubated with DMEM/F12 for a further 24 h. a The t-PERK, p-PERK, t-eIF2α, p-eIF2α, pro-caspase9, cle-caspase9, pro-caspase3, and cle-caspase3 protein levels were determined by western blot. b Results were expressed as the ratio of p-PERK and t-PERK, p-eIF2α and t-eIF2α, pro-caspase9 and β-actin, cle-caspase9 and β-actin, pro-caspase3 and β-actin, and cle-caspase3 and β-actin protein levels. Equal loading was monitored with anti-β-actin antibody. *P < 0.05, **P < 0.01, data are expressed as means ± S.D. from three independent experiments at least

Back to article page