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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Overexpression of Toll-like receptor 4 contributes to the internalization and elimination of Escherichia coli in sheep by enhancing caveolae-dependent endocytosis

Fig. 3

TLR4 overexpression promotes monocyte endocytosis. a The endocytosis of pHrodo-labelled E. coli by monocytes in the transgenic (TG) and wild-type (WT) groups was observed by fluorescence microscopy. Scale bar: 100 μm. b Flow cytometric analysis of the percentage of monocyte endocytosis of the pHrodo- labelled E. coli after 10 min and 30 min of infection. c The effect of pretreatment with the TLR4 inhibitor TAK242 on monocyte endocytosis of pHrodo- labelled E. coli. All data are presented as the mean ± SD, n ≥ 3; *P < 0.05, **P < 0.01. DMSO, dimethyl sulfoxide (control)

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891