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Table 1 Primer sets used for PCR and qPCR

From: Combination of cell signaling molecules can facilitate MYOD1-mediated myogenic transdifferentiation of pig fibroblasts

Gene

Primer sequence (5'→3')

Annealing temperature, °C

Product size, bp

FUW-TetO-pMYOD1 (ExoMYOD1)

F

CCAGGTGCTCTGAGAATTCGATA

60

114

R

CCACATAGCGTAAAAGGAGCA

  

EndoMYOD1

F

AGGGACAGGATAGAGCAGGG

60

199

R

TCAAATCTACGTCGCGGAGC

  

PAX7

F

GTGCCCTCAGTGAGTTCGAT

58

152

R

TCCAGACGGTTCCCTTTGTC

  

MYF5

F

AGTTCGGGGACGAGTTTGAG

60

232

R

TCAAACGCCTGGTTGACCTT

  

MYOG

F

GAGCTGTATGAGACATCCCCC

60

75

R

GTGGACGGGCAGGTAGTTTT

  

MHC

F

ACAGTGAAGACGGAAGCAGG

60

153

R

TGCGTAACGCTCTTTGAGGT

  

pACTB

F

CCGGGACCTGACCGACTACC

60

126

R

TCGAAGTCCAGGGCGACGTA

  

GAPDH

F

TGCTCCTCCCCGTTCGAC

60

100

R

ATGCGGCCAAATCCGTTC

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