Fig. 5

MYOD1 affected KLF4 expression. a Western blot analysis of the protein levels of KLF4 in MYOD1^OE, MYOD1^NC, and MYOD1^KO cells. b mRNA levels of adipocyte marker genes were analyzed with qPCR. c Representative images of KLF4 over-expression promoted the lipid droplet formation by oil red O staining (red). d Model of the MYOD1-mediated regulatory pathway for adipocyte differentiation. Data are shown as mean ± SD of three biological replicates. The t-test was used to analyze the statistical differences between groups. *, P < 0.05