Fig. 2

MYOD1 is a repressor of avian adipocyte differentiation. a, b MYOD1^OE cell significantly promoted MYOD1 mRNA and protein expression in ICPs. c Representative images of MYOD1^OE cells reduced the lipid droplet formation by oil red O staining on day 3. d Comparison of the lipid droplet content of MYOD1^OE and MYOD1^NC cells obtained by oil red O staining and extraction methods. e mRNA levels of adipocyte genes PPARγ, A-FABP, C/EBPα, and C/EBPβ were analyzed with qPCR. f Upper part: schematic diagram of MYOD1 exon1 region and the two targeting loci of MYOD1 sgRNA (red). Lower part: DNA sequence map around the targeting locus of the cleaved band amplified from ICPs transfected with both sgRNAs. g, h MYOD1^KO significantly reduced MYOD1 mRNA and protein expression in ICPs. i Representative images of MYOD1^KO cells promoted the lipid droplet formation by oil red O staining on day 3. j Comparison of the lipid droplet content of MYOD1^OE and MYOD1^NC cells obtained by oil red O staining and extraction methods. k mRNA levels of adipocyte marker genes were analyzed with qPCR. l Compare the fold change of known lipid biosynthesis genes in MYOD1^OE, MYOD1^NC, and MYOD1^KO cells on day 5 compared to day 0. Data are shown as mean ± SD of three biological replicates. The t-test was used to analyze the statistical differences between groups. *, P < 0.05