Dietary inclusion of a partially defatted black soldier fly (Hermetia illucens) larva meal in low fishmeal-based diets for rainbow trout (Oncorhynchus mykiss)

Table 1 Ingredients and proximate composition of the experimental diets and BSF larva meal

	BSF	BSF0	BSF3	BSF6	BSF9	BSF12	BSF15
Ingredients, %
Fishmeal^a		20.0	18.0	16.0	14.0	12.0	10.0
Hermetia illucens larva meal^b		–	3.0	6.0	9.0	12.0	15.0
Wheat gluten		13.0	13.0	13.0	13.0	13.0	13.0
Soybean meal		20.0	20.0	20.0	20.0	20.0	20.0
Swine haemoglobin		9.2	9.0	8.8	8.6	8.4	8.2
Wheat starch		23.4	22.6	21.8	21.0	20.2	19.4
Fish oil		7.0	7.0	7.0	7.0	7.0	7.0
Soybean oil		7.0	7.0	7.0	7.0	7.0	7.0
Minerals^c		0.25	0.25	0.25	0.25	0.25	0.25
Vitamins^d		0.20	0.20	0.20	0.20	0.20	0.20
Chemical composition ^e
Dry matter, g/100 g	94.0	97.2	97.2	96.9	96.8	96.9	96.6
Ash, g/100 g as fed	10.2	5.8	5.9	5.8	5.7	5.5	5.5
Crude protein, g/100 g as fed	56.9	45.6	46.1	45.6	46.0	45.7	46.1
Ether extract, g/100 g as fed	7.0	14.9	15.9	15.8	15.7	14.6	14.3
Gross energy, MJ/kg as fed	20.2	22.4	22.7	22.6	22.7	22.7	22.6
Chitin, g/100 g	6.3	–	0.18	0.37	0.56	0.75	0.93

Abbreviations: BSF Hermetia illucens. ^a Purchased from Skretting Italia S.p.A. (Località Vignetto, 17-37060 Mozzecane VR, Italy). Proximate composition (g/100 g, as fed): DM 91.0, CP 67.6, EE 8.3, Ash 16.4. ^b Provided by MUTATEC (Caumont-sur-Durance, France). ^c Mineral mixture (g/kg or mg/kg diet): dicalcium phosphate, 500 g; calcium carbonate, 215 g; sodium salt, 40 g; potassium chloride, 90 g; magnesium chloride, 124 g; magnesium carbonate, 124 g; iron sulphate, 20 g; zinc sulphate, 4 g; copper sulphate, 3 g; potassium iodide, 4 mg; cobalt sulphate, 20 mg; manganese sulphate, 3 g; sodium fluoride, 1 g (purchased from Granda Zootecnici S.r.l., Cuneo, Italy). ^d Vitamin mixture (IU/kg or mg/kg diet): DL-α-tocopherol acetate, 60 IU; sodium menadione bisulphate, 5 mg; retinyl acetate, 15,000 IU; DL-cholecalciferol, 3000 IU; Stay C Roche (vitamin C), 90 mg; thiamin, 15 mg; riboflavin, 30 mg; pyridoxine, 15 mg; vitamin B₁₂, 0.05 mg; nicotinic acid, 175 mg; folic acid, 500 mg; inositol, 1000 mg; biotin, 2.5 mg; calcium panthotenate, 50 mg (purchased from Granda Zootecnici S.r.l., Cuneo, Italy). ^e Values are reported as mean of duplicate analyses

ISSN: 2049-1891