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Table 5 The contents of ROS, MDA, PC, and GSH, and the activities of ASA, AHR, T-SOD, CAT, GPX, GST, and GR in the muscle of hybrid bagrid catfish fed experimental diets containing graded levels of Ile for 8 weeks

From: Dietary isoleucine improved flesh quality, muscle antioxidant capacity, and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish (Pelteobagrus vachelli♀ × Leiocassis longirostris♂)

Items Dietary Ile level, g/kg SEM P-values
5.0 7.5 10.0 12.5 15.0 17.5 20.0 Linear Quadratic
ROS,  % DCF fluorescence 100.00de 81.84c 61.68b 37.79a 36.13a 86.07cd 113.90e 0.72 <  0.0001 <  0.0001
MDA, nmol/mg protein 3.31b 2.29ab 1.65a 1.56a 1.98a 2.50ab 5.47c 0.27 <  0.01 <  0.0001
PC, nmol/mg protein 6.53c 6.06bc 4.40ab 3.60a 4.05a 4.52ab 5.01abc 0.31 <  0.0001 <  0.0001
GSH, mg/g protein 2.07a 2.76bc 3.25cd 3.51d 3.11cd 2.86bcd 2.37ab 0.12 0.15 <  0.0001
ASA, U/mg protein 143.59a 181.50b 240.67cd 284.19e 245.60d 209.21bc 176.86ab 4.19 <  0.0001 <  0.0001
AHR, U/mg protein 93.45a 95.32a 184.63e 163.67d 146.11c 114.58b 102.72ab 2.06 <  0.05 <  0.0001
T-SOD, U/mg protein 39.35ab 41.11ab 43.89b 46.63b 41.34ab 39.27ab 34.11a 1.23 <  0.01 <  0.0001
CAT, U/mg protein 1.47a 2.49b 3.33c 3.21c 3.15bc 2.99bc 2.94bc 0.15 <  0.0001 <  0.0001
GPX, U/mg protein 34.08 36.12 38.32 40.19 43.72 41.62 40.31 1.84 <  0.01 0.06
GST, U/mg protein 96.58bcd 100.03bcd 108.74d 105.23cd 93.68abc 89.56ab 82.48a 1.81 <  0.0001 <  0.0001
GR, U/mg protein 5.21a 6.94bc 8.02cd 9.76e 9.86e 9.55de 5.62ab 0.27 < 0.0001 < 0.0001
  1. Values are mean ± SEM of three replicate groups with 6 fish in each group. Mean values with the different superscripts in the same row are significantly different (P < 0.05)