Table 5 The contents of ROS, MDA, PC, and GSH, and the activities of ASA, AHR, T-SOD, CAT, GPX, GST, and GR in the muscle of hybrid bagrid catfish fed experimental diets containing graded levels of Ile for 8 weeks

ROS,  % DCF fluorescence

100.00^de

81.84^c

61.68^b

37.79^a

36.13^a

86.07^cd

113.90^e

0.72

<  0.0001

<  0.0001

MDA, nmol/mg protein

3.31^b

2.29^ab

1.65^a

1.56^a

1.98^a

2.50^ab

5.47^c

0.27

<  0.01

<  0.0001

PC, nmol/mg protein

6.53^c

6.06^bc

4.40^ab

3.60^a

4.05^a

4.52^ab

5.01^abc

0.31

<  0.0001

<  0.0001

GSH, mg/g protein

2.07^a

2.76^bc

3.25^cd

3.51^d

3.11^cd

2.86^bcd

2.37^ab

0.12

0.15

<  0.0001

ASA, U/mg protein

143.59^a

181.50^b

240.67^cd

284.19^e

245.60^d

209.21^bc

176.86^ab

4.19

<  0.0001

<  0.0001

AHR, U/mg protein

93.45^a

95.32^a

184.63^e

163.67^d

146.11^c

114.58^b

102.72^ab

2.06

<  0.05

<  0.0001

T-SOD, U/mg protein

39.35^ab

41.11^ab

43.89^b

46.63^b

41.34^ab

39.27^ab

34.11^a

1.23

<  0.01

<  0.0001

CAT, U/mg protein

1.47^a

2.49^b

3.33^c

3.21^c

3.15^bc

2.99^bc

2.94^bc

0.15

<  0.0001

<  0.0001

GPX, U/mg protein

34.08

36.12

38.32

40.19

43.72

41.62

40.31

1.84

<  0.01

0.06

GST, U/mg protein

96.58^bcd

100.03^bcd

108.74^d

105.23^cd

93.68^abc

89.56^ab

82.48^a

1.81

<  0.0001

<  0.0001

GR, U/mg protein

5.21^a

6.94^bc

8.02^cd

9.76^e

9.86^e

9.55^de

5.62^ab

0.27

< 0.0001

< 0.0001