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Table 3 Muscle fibers cellularity, % frequency of hybrid bagrid catfish fed diets with graded levels of Ile for 8 weeks1

From: Dietary isoleucine improved flesh quality, muscle antioxidant capacity, and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish (Pelteobagrus vachelli♀ × Leiocassis longirostris♂)

Items2

Dietary Ile levels, g/kg

SEM

P-values

5.0

7.5

10.0

12.5

15.0

17.5

20.0

Linear

Quadratic

d ≤ 20 μm

24.07a

26.18ab

25.12ab

33.12c

29.21bc

23.81a

24.04a

0.51

0.74

<  0.0001

20 < d < 50 μm

66.91d

63.42cd

58.69bc

48.93a

55.56b

64.31cd

66.42d

0.80

0.52

<  0.0001

d ≥ 50 μm

9.03a

10.40a

16.19bc

18.75c

15.24b

11.89ab

10.02a

0.41

<  0.05

<  0.0001

  1. 1 Values are means ± SEM of three replicate groups with 2 fish in each group. Mean values with the different superscripts in the same row are significantly different (P < 0.05)
  2. 2 d, diameter of muscle fibers