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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: Dietary isoleucine improved flesh quality, muscle antioxidant capacity, and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish (Pelteobagrus vachelli♀ × Leiocassis longirostris♂)

Fig. 5

Effect of dietary Ile on the PI3K-AKT-TOR signaling pathway in hybrid bagrid catfish muscle fed diets containing graded levels of Ile. (a) The mRNA levels of PI3K, AKT, TOR, S6K1, and 4E-BP1; (b) Protein abundances of p-AKT (Ser473), AKT, and ratio of p-AKT and AKT; (c) Protein abundances of p-TOR (Ser2448), TOR, and ratio of p-TOR and TOR; (d) Protein abundances of p-S6K1 (Thr421/Ser424), S6K1, and ratio of p-S6K1 and S6K1. Data represent means ± SEM, of three replicates. Values having different letters are significantly different (P < 0.05)

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