Fig. 5

Effect of dietary Ile on the PI3K-AKT-TOR signaling pathway in hybrid bagrid catfish muscle fed diets containing graded levels of Ile. (a) The mRNA levels of PI3K, AKT, TOR, S6K1, and 4E-BP1; (b) Protein abundances of p-AKT (Ser473), AKT, and ratio of p-AKT and AKT; (c) Protein abundances of p-TOR (Ser2448), TOR, and ratio of p-TOR and TOR; (d) Protein abundances of p-S6K1 (Thr421/Ser424), S6K1, and ratio of p-S6K1 and S6K1. Data represent means ± SEM, of three replicates. Values having different letters are significantly different (P < 0.05)