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Table 2 Sequence of primers used for qPCR

From: Beneficial effects of a decreased meal frequency on nutrient utilization, secretion of luteinizing hormones and ovarian follicular development in gilts

Genes

Forward

Reverse

β-ACTIN

GGCCGCACCACTGGCATTGTCAT

AGGTCCAGACGCAGGATGGCG

FSHR

TCACAGTCCCTCGGTTCCTT

AGCATCACAGCCTGCTCCA

LHCGR

ATGGGGCTCTACCTGCTACTCA

GAGCCACCCTCCAAGCATAA

GDF9

CTCTGCCTCTTCCTCCTCCACTG

GGTGAATGAGTACGGTGCTCTTGG

BMP15

ACCATGCCATCATTCAGAGCCTTG

CGTTGGTCTCAATCAGGAGGATGC

CYP11A1

GGCTCCAGAGGCCATAAAGA

ACTCAAAGGCGAAGCGAAAC

STAR

GACTTTGTGAGTGTCGGCTGTA

ATCCCTTGAGGTCAATGCTG

3β-HSD

CACTGACCTGGGCTGATGAC

GTGGCGAGAAGCAGACAAGA

CYP17A1

TCCAAGCCAAGACGAAC

TTTACCACAGAGGCAGAAG

  1. Abbreviations: FSHR follicle-stimulating hormone receptor, LHCGR luteinizing hormone/chorionic gonadotropin receptor, GDF9 growth differentiation factor 9, BMP15 bone morphogenetic protein 15, CYP11A1 cytochrome P450 family 11 subfamily A member 1, STAR steroidogenic acute regulatory protein, 3βHSD 3 β-hydroxysteroid dehydrogenase, CYP17A1 cytochrome P450 family 17 subfamily A member 1
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891