Table 3 List of primer sequences used for quantitative real-time PCR
Gene symbol | Primer sequence (5′ → 3′) | |
|---|---|---|
Forward | Reverse | |
CEBPA | CCCACCTGCAGTACCAGATC | TCTTTTTGGATTTGCCGCGG |
CEBPB | CGCCCGCCTTTAAATCCATG | GGGCTGAAGTCAATGGCTCT |
CEBPD | ACTTCTACGACGCCAAGGTG | CTCTCGTCCTCGTACATGGC |
CEBPG | CCCACAGCTAACGTGTCAGT | GGACGGGCTCTTCTTTGACA |
CEBPZ | CGCTGTTCACAGTCTCCACT | GGACGCTGTGAGAAAGACCA |
SOX2 | AAACCGAGCTGAAACCTCCC | TGTGCATCTTCGGGTTCTCC |
SOX3 | CGGCTCAGCAGACTCGATAC | TCGCCGTGGCTTAAGAACTT |
POU5F3 | TGAAGGGAACGCTGGAGAGC | ATGTCACTGGGATGGGCAGAC |
TP53 | CCGTGGCCGTCTATAAGAAA | ACAGCACCGTGGTACAGTCA |
NANOG | AGTGGCAGAGTCTGGGGTAT | ACTACTACTGGCCCTCTCCG |
GAPDH | GGTGGTGCTAAGCGTGTTAT | ACCTCTGTCATCTCTCCACA |