Fig. 4

Negative regulation of chicken NANOG (cNANOG) gene expression by TP53 in chicken primordial germ cells (PGCs). a Prediction of transcription factor (TF)-binding sites in the cNANOG promoter region from − 250 to −210 bp. b Mutation analysis of putative TP53-binding sites in PGCs. c Luciferase activity of pNL-NANOG − 250/+ 70 and TP53-binding site-mutated (pNL-NANOG − 250/+ 70 TP53 mutation) vectors. pNL1.2-Basic was used as a control. Significant differences are indicated as ns (no significance) and *** P < 0.001. Error bar represent the SEs for five replicate reactions