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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Differential transcriptional regulation of the NANOG gene in chicken primordial germ cells and embryonic stem cells

Fig. 2

Promoter variants reduce activity of the chicken NANOG (cNANOG) gene in a cell type-dependent manner. a Schematic diagram of deletion of the cNANOG gene promoter (− 3550/+ 70 bp). Relative luciferase activity in chicken primordial germ cells (PGCs) (b), chicken embryonic stem cells (ESCs) (c), and DF-1 cells (d). Luciferase activity was normalized against firefly luciferase expression (pNL1.2-Basic) to control for variation in the transfection efficiency. Significant differences are indicated as ns (no significance), ** P < 0.01, and *** P < 0.001. Error bar represent the SEs for three replicate reactions

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