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Table 1 Primer sequences of the genes analyzed

From: Transcription factor EB (TFEB)-mediated autophagy protects bovine mammary epithelial cells against H2O2-induced oxidative damage in vitro

Gene

Primer sequences (5′→3′)

Length, bp

Efficiency (E%)

TFEB

F TGCTGACCCCAGATCCAACT

R CCCAAACCTGCTTGATCACC

76

101

β-actin

F GCCCTGAGGCTCTCTTCCA

R GCGGATGTCGACGTCACA

101

97

GAPDH

F GTCTTCACTACCATGGAGAAGG

R TCATGGATGACCTTGGCCAG

197

103

  1. The efficiency was determined as [E = (10–1/slope−1) × 100%]