Fig. 4

Effects of TFEB knockdown on autophagy in MAC-T cells. a mRNA abundance of TFEB. MAC-T cells were transfected with TFEB siRNA for 48 h. b Western blot analysis of LC3-II and p62. MAC-T cells were transfected with TFEB siRNA for 48 h and/or treated with H₂O₂ (0.5 mmol/L) for another 24 h. c Protein abundance of LC3-II. d Protein abundance of p62. e Representative images of autophagosomes (yellow puncta) and autolysosomes (red puncta), scale bar = 25 μm. MAC-T cells were co-transfected with the recombinant adenovirus mRFP-GFP-LC3 and TFEB siRNA for 48 h, and/or treated with H₂O₂ (0.5 mmol/L) for another 24 h. The data presented are the mean ± SEM. Different superscript lowercase letters in bar charts represent significant difference (P < 0.05)