Fig. 2

Effects of inhibition of autophagy on oxidative damage of MAC-T cells. a Representative images of autophagosomes (yellow puncta) and autolysosomes (red puncta), scale bar = 25 μm. MAC-T cells were transfected with recombinant adenovirus mRFP-GFP-LC3 for 48 h and then pre-treated with BafA1 (100 nmol/L) or DMSO (vehicle control; Veh) in amounts equal to that present in BafA1 cultures for 12 h, and treated with H₂O₂ (0.5 mmol/L) for another 24 h. b Western blot analysis of LC3-II and p62. MAC-T cells were pre-treated with BafA1 (100 nmol/L) or DMSO (Veh) in amounts equal to that present in BafA1 cultures for 12 h, and/or treated with H₂O₂ (0.5 mmol/L) for another 24 h. c Protein abundance of LC3-II. d Protein abundance of p62. e ROS content. f The % of apoptotic cells. The data presented are the mean ± SEM. Different superscript lowercase letters in bar charts represent significant difference (P < 0.05)