Fig. 1

Effects of H₂O₂ on the oxidative stress and autophagy in MAC-T cells. a ROS content. MAC-T cells were treated with 0, 0.25, 0.5 or 1 mmol/L H₂O₂ for 24 h. b Western blot analysis of LC3-II and p62. c Protein abundance of LC3-II. d Protein abundance of p62. e Representative images of autophagosomes (yellow puncta) and autolysosomes (red puncta), scale bar = 25 μm. MAC-T cells were transfected with the recombinant adenovirus mRFP-GFP-LC3 for 48 h, and/or treated with H₂O₂ (0.5 mmol/L) for another 24 h. The data presented are the mean ± SEM. Different superscript lowercase letters in bar charts represent significant difference (P < 0.05)