Skip to main content
Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Effects of age on immune function in broiler chickens

Fig. 3

The third immune development pattern of broiler chickens housed in laminated cages is Up-Up. The levels of NO (a), C3 (b), C4 (c), IL-2 (d), IFN-γ/IL-4 (e), IgG (f) in serum and sIgA in ileum mucosa (p) were analyzed by ELISA kit. The frequencies of Tc cells (g) of peripheral blood lymphocytes were analyzed by flow cytometry. The spleen index was analyzed by weighing (h). The mRNA levels of NF-κB in spleen (i), MHC-II (j), CD3d (k), TCRβ subunit (l), TCRζ subunit (m), IFN-γ (n) and pIgR (o) in ileum were analyzed by RT-PCR. Different small letters indicated significant difference (P < 0.05)

Back to article page