Table 3 Primer and probe sequences used for real-time PCR
Items | Primer and probe sequence (5´→3´) | Product length, bp |
|---|---|---|
Total bacteria | F: ACTCCTACGGGAGGCAGCAG R: ATTACCGCGGCTGCTGG | 200 |
Lactobacillus | F: GAGGCAGCAGTAGGGAATCTTC R: CAACAGTTACTCTGACACCCGTTCTTC | 126 |
P: AAGAAGGGTTTCGGCTCGTAAAACTCTGTT | Â | |
Bifidobacterium | F: CGCGTCCGGTGTGAAAG R: CTTCCCGATATCTACACATTCCA | 121 |
P: ATTCCACCGTTACACCGGGAA | Â | |
Bacillus | F: GCAACGAGCGCAACCCTTGA R: TCATCCCCACCTTCCTCCGGT | 92 |
P: CGGTTTGTCACCGGCAGTCACCT | Â | |
Escherichia coli | F: CATGCCGCGTGTATGAAGAA R: CGGGTAACGTCAATGAGCAAA | 96 |
P: AGGTATTAACTTTACTCCCTTCCTC | Â |