Table 2 Nucleotide sequences of primers used to measure targeted genes (Exp. 2)
Gene symbols | Nucleotide sequence of primers (5´→3´) | Product length, bp | Accession No. |
|---|---|---|---|
β-actin | F: TCTGGCACCACACCTTCT R: TGATCTGGGTCATCTTCTCAC | 78 | XM_021086047.1 |
TLR2 | F: ACGTATCCATCAATGAACACTGC R: AAGGGTGCAGTCATCAAACTC | 146 | NM_213761.1 |
TLR4 | F: TCAGTTCTCACCTTCCTCCTG R: GTTCATTCCTCACCCAGTCTTC | 121 | GQ503242.1 |
TLR6 | F: TCTCATGGCACAGCGAACTT R: ACATCATCCTCTTCAGCGACT | 123 | NM_213760.2 |
MyD88 | F: GGTGCCAGGCAGGACATC R: GGCAGCTGGAACAGACCAA | 68 | NM_001099923.1 |
IL-6 | F: GACAAAGCCACCACCCCTAA R: CTCGTTCTGTGACTGCAGCTTATC | 69 | M80258.1 |
IL-1β | F: TCTGCCTGTACCCCAACTG R: CCAGGAAGACGGGCTTTTG | 63 | NM214055.1 |
TNF-α | F: CGTGAAGCTGAAAGACAACCAG R: GATGGTGTGAGTGAGGAAAACG | 121 | EU682384.1 |
IL-10 | F: GACCAGATGGGCGACTTGTT R: TGCCTTCGGCATTACGTCTT | 120 | NM_214041.1 |
ZO-1 | F: GAGGATGGTCACACCGTGGT R: GGAGGATGCTGTTGTCTCGG | 169 | XM_021098896.1 |
Claudin-1 | F: TCAATACAGGAGGGAAGCCAT R: ATATTTAAGGACCGCCCTCTCC | 91 | NM_001244539.1 |
Occludin | F: CAGGTGCACCCTCCAGATTG R: TGGACTTTCAAGAGGCCTGG | 111 | NM_001163647.2 |