Effects of full replacement of dietary fishmeal with insect meal from Tenebrio molitor on rainbow trout gut and skin microbiota

Table 1 Main ingredients and proximate composition of the diets

Item	TM 0	TM 100
Ingredients, %
Fishmeal 65 (Peruvian)	20	-
Tenebrio molitor larvae meal	-	20
Soy protein concentrate	18	18
Wheat gluten	7.75	7.06
Corn gluten	8	8
Soybean meal (48%)	7	7
Wheat meal	14.23	13.8
Sardine oil	4.3	4.1
Soybean oil	8.6	8.2
Rapeseed oil	8.6	8.2
Soy lecithin	0.5	0.5
Vitamin^a and mineral premix^b	1	1
Antioxidant	0.2	0.2
Sodium propionate	0.1	0.1
Monocalcium phosphate	0.52	1.73
L-Arginine	-	0.1
L-Lysine	-	0.6
L-Tryptophan	0.05	0.12
DL-Methionine	0.15	0.3
Celite®	1	1
Proximate composition, % as fed
Dry matter	93.77	94.41
Crude protein	42.08	44.25
Ether extract	22.63	22.36
Ash	7.57	5.6
Chitin	-	1.49
Nitrogen-free extract^c	21.49	20.71
Gross energy, MJ/kg as fed^d	22.24	22.55

This table has been modified from previously published data in Chemello et al. [36]
^aVitamin mixture (IU or mg per kg diet): DL-αtocopherolacetate, 60 IU; sodium menadione bisulfate, 5 mg; retinylacetate, 15,000 IU; DL-cholecalciferol, 3000 IU; thiamin, 15 mg; riboflavin, 30 mg; pyridoxine, 15 mg; vitamin B₁₂, 0.05 mg; nicotinic acid, 175 mg; folic acid, 500 mg; inositol, 1000 mg; biotin, 2.5 mg; calcium panthotenate, 50 mg; choline chloride, 2000 mg (Granda Zootecnici, Cuneo, Italy)
^bMineral mixture (g or mg per kg diet): bicalcium phosphate 500 g, calcium carbonate 215 g, sodium salt 40 g, potassium chloride 90 g, magnesium chloride 124 g, magnesium carbonate 124 g, iron sulfate 20 g, zinc sulfate 4 g, copper sulfate 3 g, potassium iodide 4 mg, cobalt sulfate 20 mg, manganese sulfate 3 g, sodium fluoride 1 g (Granda Zootecnici, Cuneo, Italy)
^cCalculated as 100 − (crude protein + ether extract+ ash + chitin)
^dDetermined by calorimetric bomb

ISSN: 2049-1891